Figure 8. Compound 1 augments ERK1/2 and p38 activation in vivo.
ERK1/2 and p38 phosphorylation in spleenocytes of mice injected (i.p.) with compound 1 or vehicle. Total lysates from spleen were probed with phospho-specific antibodies against pT202/Y204-ERK1/2 (pERK1/2), pT180/Y182-p38 (pp38), pS473-AKT1 (pAKT), and pS23-HePTP (pHePTP). Phosphorylation levels were normalized to total protein, then to GAPDH as a loading control. (*p<0.05, **p<0.01, n=4).