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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Nat Chem Biol. 2012 Jan 22;8(3):253–261. doi: 10.1038/nchembio.770

Figure 4.

Figure 4

CREB glycosylation at Ser40 represses dendritic and axonal growth. (a) Relative total dendrite lengths of cortical neurons expressing GFP, WT CREB or S40A CREB. (For each condition, thirty dendrites were measured in each of three independent experiments, *P < 0.001; NS, not significant). (b) Relative axon lengths of OGT floxed cortical neurons expressing GFP, Flag-tagged OGT, or Cre recombinase (OGT KO). Neurons were also transfected with scramble or CREB siRNA as indicated. (For each condition, thirty axons were measured in each of three independent experiments, *P < 0.001). (c) Relative dendrite lengths of unstimulated neurons transfected with WT or S40A CREB, Wnt2 signaling inhibitors (Wnt2 siRNA, Dickkopf-1 (Dkk-1), Ncad(intra)) or CRTC1 siRNA as indicated. The BDNF signaling inhibitor TrkB-Fc was added in solution to neurons. (For each condition, thirty dendrites were measured in each of three independent experiments, *P < 0.001). Lengths are shown relative to unstimulated GFP-expressing neurons in (A). Error bars, means, and s.e.m.