Dynamic mechanical responses of A431 cells to stimulation with four different doses of EGF: 0, 10 nM, 20 nM, and 40 nM. A 30-min baseline was established for each measurement prior to the addition of EGF. Each data point of the plots was determined from an average of 10 force displacement curves recorded at the time for a period of 10 min. Trend lines were used to connect each data point to illustrate the trend for each response curve. All mechanical responses in (A) energy dissipation, (B) Young’s modulus, (C) hysteresivity show a dose-dependence of EGF. The responses in the presence of 10 nM, 20 nM, and 40nM of EGF show a progressive increase over the time of the EGF stimulation.