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. Author manuscript; available in PMC: 2013 Feb 16.
Published in final edited form as: Cell Host Microbe. 2012 Jan 26;11(2):194–204. doi: 10.1016/j.chom.2012.01.004

Figure 1. Vpx from diverse primate lentiviruses degrade SAMHD1.

Figure 1

(A) The ability of Vpx and Vpr to degrade SAMHD1 was assayed by western blot analysis of HA-epitope tagged SAMHD1 from respective primate species co-transfected with 3xFLAG-epitope tagged Vpr or Vpx constructs as indicated. Actin was probed as a loading control. Indicated Vpr and Vpx constructs were expressed in the presence of human SAMHD1 (left) or chimpanzee SAMHD1 (right).

(B) Similar western blots as in (A) are shown, analyzing rhesus macaque SAMHD1 (left) and red-capped mangabey (RCM) SAMHD1 (right) expression in the presence of indicated Vpr and Vpx constructs.

(C) Similar western blots as in (A) are shown, analyzing mandrill SAMHD1 expression in the presence of indicated Vpr and Vpx from SIVmnd1 or SIVmnd2.