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. Author manuscript; available in PMC: 2013 Feb 16.
Published in final edited form as: Cell Host Microbe. 2012 Jan 26;11(2):194–204. doi: 10.1016/j.chom.2012.01.004

Figure 4. SAMHD1 positive selection residues map to Vpx sensitivity.

Figure 4

(A) Alignment of N-terminal and SAM domain regions from indicated primates. Symbols (circle on a stick) represent the positively selected residues marked on the SAMHD1 domains. Sites 46 and 69 which displayed highly significant signals of positive selection are boxed in the alignment. Stars represent the codons under positive selection with strong support (Figure S3A, S3B and S3C). The N terminal region of SAMHD1 from grey mouse lemur is included to represent amino acid residues encoded by a distantly related prosimian primate.

(B) Expression of mandrill, AGM and AGM point mutants (AGM D46G and AGM Q69R) were analyzed by western blot, in the presence or absence of SIVmnd2 Vpx expression.

(C) Western blot analysis of HA-immunoprecipitated SAMHD1 for FLAG-epitope tagged SIVmnd2 Vpx association. Cells were treated with 25μM MG-132 for 12 hours prior to immunoprecipitation. Heavy chain (Hc) is shown as a loading control.

(D) Western blot shows expression of SAMHD1 from mandrill and mandrill-derived mutations (Mnd G46D, Mnd R69Q, and Mnd G46D, R69Q) in the presence or absence of SIVmnd2 Vpx.