Table I.

Species-specific SAMHD1 degradation by Vpr and Vpx.

SAMHD1	HIV-2 Rod9 Vpx	HIV-2 7312a Vpx	SIVmac Vpx	SIVdeb Vpr	SIVmus Vpr	SIVagm Gri Vpr	SIVagm Ver Vpr	SIVrcm Vpx	SIVmnd2 Vpx
Human	+	+	+	+	+	-	-	-	-
Rhesus	-	+	+	+	+	-	+	+	+
RCM	-	+	+	+	+	-	+	+	+
Mandrill	-	+	+	+	+	-	+	+	+
AGM	-	+	+	+	+	+	+	+	-
Deb	+	+	+	+	+	-	+	+	+
SM	-	+	+	+	+	-	+	-	+

The table summarizes results of western blot analyses of SAMHD1 degradation phenotype by indicated Vpr and Vpx across a panel of primate SAMHD1. “+” indicates combinations that resulted in marked decrease in SAMHD1 levels. “-” indicates combinations that had no detectable changes in SAMHD1 levels. The following Vpr proteins from HIV-1 Lai, HIV-1 Q23-17, HIV-2 Rod9, HIV-2 7312a, SIVcpz 3.1, SIVcpz 2.69, SIVmac239, SIVrcm, SIVmnd1, SIVmnd2, and SIVolc – were inactive against their host species’ as shown in Figure 1 and 2 were also unable to degrade the panel of primate species’ SAMHD1 (Data not shown). The AGM SAMHD1 tested is from the Vervet subspecies matching the SIVagmVer 9648 host strain; SAMHD1 from the Tantalus subspecies was found to be heterozygous for a second allele that was resistant to all HIV/SIV Vpr and Vpx tested (data not shown).