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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Dev Dyn. 2012 Jan 31;241(3):583–594. doi: 10.1002/dvdy.23740

Figure 4. Claudin 3 is broadly expressed in developing pancreatic epithelia but becomes restricted to ductal and acinar cells after birth.

Figure 4

(A) Claudin 3 is co-expressed with Pdx1 (arrow) in multipotent progenitors of E10.5 pancreata. (B) At this stage, claudin 3 (red; arrow) localizes to the lateral membrane of pancreatic cells. (C) At E12.5, following polarization of the pancreatic primordium, claudin 3 (red; arrows) co-localizes with ZO-1 (green) at tight junctions in primitive ducts. (D,E) Claudin 3 and ZO-1 coexpression is noticed towards the lumen of the emerging pancreatic ductal network at E15.5 (arrows in D), and in ductal epithelial cells at E18.5 (arrows in E). (F) Claudin 3 is expressed at high levels in ductal cells (Opn+ cells; arrows), and at lower levels in acinar cells (arrowhead), in P5 pancreata. (G,H) Claudin 3 co-localizes with ZO-1 in both ductal (arrows in G) and acinar (arrows in H) cells in P5 pancreata. Asterisk in G indicates an autofluorescent red blood cell. B–H are confocal images (cell nuclei were stained with DAPI [blue]). Scale bars: 25 μm (A), 20 μm (F), 10 μm (B–E,G), 5 μm (H).