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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Protein Expr Purif. 2011 Dec 14;82(1):116–124. doi: 10.1016/j.pep.2011.11.015

Fig 1.

Fig 1

(A) Schematic diagram of the recombinant proteins used in this study. Top, wild-type and mutant DsbC produced by pBA2219 and pBA2283, respectively. Middle, DsbC/DsbA fusion protein produced by pBA2285. Bottom, MBP-proMeCPAHis6RP fusion protein produced by pBA2257. In-frame amino acid extensions and interdomain linkers arising from Gateway cloning artifacts are indicated by lines. (B) Amino acid sequence of proMeCPA-His6RP. The thermolysin cleavage site is indicated by an arrow. The two pairs of cysteine residues that form disulfide bonds [24] are enclosed by boxes and linked together by lines. The C-terminal His6PR tag is underlined.