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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Protein Expr Purif. 2011 Dec 14;82(1):116–124. doi: 10.1016/j.pep.2011.11.015

Fig 4.

Fig 4

Comparison of MeCPA activity obtained from three different protocols. (◆), Buffer only control; (■), Clarified cells lysate was immediately subjected to amylose affinity chromatography, digested with thermolysin and assayed; (▲), Clarified cell lysate was incubated overnight at 4 C, then subjected to amylose affinity chromatography, digested immediately afterward by thermolysin and assayed; (●), Clarified cell lysate was immediately subjected to amylose affinity chromatography and then incubated overnight prior to thermolysin digestion and activity assay.