. Author manuscript; available in PMC: 2013 Mar 1.

Published in final edited form as: Protein Expr Purif. 2011 Dec 14;82(1):116–124. doi: 10.1016/j.pep.2011.11.015

Table 1.

Purification of MeCPA-His₆PR

Purification step	Total protein/L^a	Purification-fold	Purity^b	Yield/L
Bacterial lysate	2.7 g	1	0	2.7 g
Amylose	2.7 g	19.3	45.2 %	0.14 g
Thermolysin digestion	0.12 g	1	1.4 %	0.12 g
IMAC	0.12 g	171.4	96.5 %	0.70 mg
Ion-exchange	0.70 mg	1.43	>98.0 %	0.49 mg
Gel filtration	0.49 mg	1.40	>98.0 %	0.34 mg

Protein concentration and total amount of protein was determined by measuring its absorbance at 280 nm.

Purity of MBP-proMeCPAHis₆PR (up until thermolysin digestion) and MeCPA- His₆PR (after thermolysin digestion) was estimated by SDS-PAGE using ImageQuant software.