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Fast separation of 11 peptides in 6 minutes with 2.9 minimum resolution. Column: 2.1 × 100 mm Halo Peptide ES-C18; Flow rate: 1.25 mL/min; Temperature 45 °C; Gradient: 10 - 54% B from 0-4 min. 54-100% B from 4-6 min; A: water/0.1% TFA; B: 80/20 acetonitrile/water/0.1% trifluoroacetic acid; Sample: 15 μL of 11 synthetic peptides, 50 ng each; Detection: 215 nm.
