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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Front Neuroendocrinol. 2011 Nov 4;33(1):85–104. doi: 10.1016/j.yfrne.2011.10.001

Figure 8. Decreased expression of ER, and increased expression of ER ubiquitination and bindings with CHIP protein in hippocampal CA1 in aged (24 month) rats compared to young (3 month) rats.

Figure 8

(A) Hippocampal homogenates from young and aged F344 rats were subjected to Western blot analysis to test the expression profile of ERα protein (a, b). (B, C) Hippocampal CA1 protein samples were immunoprecipitated (IP) with anti-ERα antibody and probed with anti-CHIP, anti-ubiquitin (Ub) or anti-ERα antibody. CHIP protein expression did not change in young versus aged rats. (D) ERβ protein expression and its bindings with ubiquitin and CHIP were examined by Western blotting and co-IP analyses, respectively. All the data are shown as means ± SE from independent animals (n = 5–7) and expressed as fold vs. group of young animals. * and ** denote P < 0.05 and P < 0.001 respectively, compared to group of young animals. Adapted from [202].