Fig. 3.
WASP family proteins show distinct patterns of expression in follicle cells, as well as in the oocyte. A-Aiii: Surface Z-projection showing the asymmetric localization of Whamy in follicles cells (α-Whamy, green; DAPI, blue). (Ai-Aiii) Higher magnification views of regions indicated in A. B-E”: Surface Z-projections of egg chambers stained with α-Whamy (green or grayscale) and alpha-tubulin to visualize microtubules (MTs) (B-B”, red or grayscale), the golgi protein Lava lamp (C-D”, red or grayscale), or the mitochondria marker MitoTracker (E-E”, red or grayscale). DAPI (blue) is included to visualize nuclei in D”. F-I: Surface Z-projections of stage 8, 10, 12 and 14 egg chambers stained with α-Wash (F), α-WASp (G), α-SCAR (H), or α-Whamy (I). Scale bars: A-Aiii, B-B”, E-E”: 10µm; C-D”: 100µm, F-I, 20µm.