. 2012 Feb 24;3:20. doi: 10.3389/fphar.2012.00020

Table 1.

Levels of hematopoietic growth factors in Gl-PS–SCM and Gl-PS–BMSCM.

Concentration of Gl-PS (μg/ml)	G-CSF	GM-CSF	IL-1β	IL-6	SCF
Gl-PS–SCM
0	0.0 ± 0.0	5.9 ± 2.3	12.7 ± 4.8	80.3 ± 6.4	0.0 ± 0.0
25	472.6 ± 53.7^a	15.0 ± 3.4^a	99.5 ± 12.6^a	289.2 ± 22.4^a	0.0 ± 0.0
50	634.8 ± 82.1^a	26.7 ± 4.9^a	169.0 ± 14.7^a	439.8 ± 41.0^a	0.0 ± 0.0
100	801.3 ± 99.2^a	38.7 ± 4.6^a	281.4 ± 22.5^a	635.7 ± 77.0^a	0.0 ± 0.0
200	1070.2 ± 123.0^a	50.2 ± 6.3^a	479.5 ± 40.7^a	857.3 ± 104.6^a	0.0 ± 0.0
Gl-PS–BMSCM
0	8.0 ± 3.1	3.2 ± 1.5	5.3 ± 3.1	38.2 ± 5.3	20.3 ± 4.1
25	20.8 ± 4.8^a	11.5 ± 2.4^a	9.5 ± 3.6	155.7 ± 6.9^a	43.2 ± 7.5^a
50	38.8 ± 7.3^a	18.7 ± 4.8^a	7.8 ± 3.1	298.8 ± 15.2^a	76.0 ± 10.7^a
100	71.2 ± 11.2^a	49.2 ± 10.3^a	8.2 ± 5.2	797.0 ± 33.3^a	92.5 ± 13.6^a
200	101.7 ± 20.5^a	50.7 ± 17.0^a	9.0 ± 4.9	922.2 ± 72.2^a	155.6 ± 19.5^a

Splenocytes (1 × 10⁷) or bone marrow stromal cells (1 × 10⁶) were cultured with Gl-PS (0–200 μg/ml) for 48 h. Gl-PS-splenocyte-conditioned medium (Gl-PS–SCM) and conditioned medium of bone marrow stromal cells (BMSCM) induced by Gl-PS were collected to measure cytokines by ELISA.

^aP < 0.05 versus control (concentration of Gl-PS: 0 μg/ml).