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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: J Immunol. 2012 Jan 27;188(5):2380–2386. doi: 10.4049/jimmunol.1102454

Figure 2. Supplementing IKKα or p52 KO cells with recombinant CXCL12/SDF-1 rescues their migration responses to HMGB1.

Figure 2

IKKα conditional KO primary MΦ (A) or immortalized IKKα or p52 KO MEFs (B) with or without supplementation by 5 ng/ml of recombinant CXCL12/SDF-1 were subjected to HMGB1 migration assays as indicated; and PDGF served as a positive migration control for the KO cells. Experiments were performed 3–5 times (3× for WT and 5× for all KO samples) each in duplicate. Mean values of WT and IKKα KO MΦ basal migration distances towards serum free media controls were 30 ± 1.0 and 31 ± 2.5 μm respectively. Mean values of IKKα KO and p52 KO MEF basal migration towards serum free media controls were 11 ± 4.0 and 6 ± 2.0 cells per HPF respectively. Basal migration in serum free media with independent preparations of WT MΦ and MEFs routinely varied between 20 to 25 microns and 15 to 20 cells per HPF respectively over the 3 hour chemotaxis assay. Error bars are standard error of the mean. P values were determined by one way ANOVA with Tukey's post test as follows: ***p = <0.001; *p = <0.05; ns = not significant.