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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: J Immunol. 2012 Jan 27;188(5):2380–2386. doi: 10.4049/jimmunol.1102454

Figure 3. NF-κB p52 KO cells engineered to express near physiological levels of CXCL12 migrate in response to HMGB1 akin to WT cells.

Figure 3

(A) CXCL12 ELISA assays of supernatants of WT MEFs, NF-κB p52 KO MEFs and FACS purified CXCL12-GFP expressing p52 KO MEFs; (B) Boyden chamber cell migration assays of WT MEFs compared to p52 KO and p52 KO/CXCL12-GFP cells. ELISA assays (A) were performed 6 times. Migration assays (B) were performed 3–4 times (3× for WT and 4× for p52 KO and p52 KO/CXCL12-GFP cells) each in duplicate. Mean values of WT, p52 KO and p52 KO + CXCL12 MEF basal migration towards serum free media controls were 7 ± 1.0, 11 ± 2.1 and 9 ± 1.8 cells per HPF respectively. P values were derived by one way ANOVA with Tukey's post test as follows: ***p = <0.001; ns = not significant.