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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: J Immunol. 2012 Jan 27;188(5):2380–2386. doi: 10.4049/jimmunol.1102454

Figure 5. Exposure of WT MΦ to either low doses of AMD3100 or to an anti-CXCR4 neutralizing antibody show that CXCR4 activity is essential for cell migration in response to HMGB1.

Figure 5

(A) Effects of different doses of AMD3100 on WT MΦ migrations as indicated. (B) WT MΦ migrations were done in the presence and absence of a neutralizing anti-CXCR4 monoclonal antibody (15 μg/ml) as indicated. Migration results with 15/μg/ml of a rat IgG2B isotype control antibody are also shown in Panel B. Experiments were performed 3–5×, each in duplicate. Mean values of WT MΦ basal migration distances towards serum free media controls were 24 ± 4.0 μm and 30 ± 1.2 μm for rat IgG2b controls. All error bars are standard error of the mean. P values were determined by one way ANOVA with Tukey's post test as follows: ***p = <0.001.