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. Author manuscript; available in PMC: 2013 Feb 21.
Published in final edited form as: Curr Biol. 2012 Feb 2;22(4):278–288. doi: 10.1016/j.cub.2012.01.014

Figure 6. Rho-dependent activities of myosin-IXA.

Figure 6

(A) Partial rescue of cell-cell contact stability in myosin-IXA-depleted cells (smartpool siRNA) after inhibiting Rho-dependent acto-myosin contractility, with Rho-kinase inhibitor Y-27632 (5μM). Cells with swollen lamellipodia (arrowheads) and rounded cell body in myosin-IXA-depleted cells become flattened (arrows) after treatment with Y-27632 (Movie S8). Time is in min:sec. Bar = 30 μm. (B) Quantification of rescue of cell-cell contact stability by Y-27632. 2 independent experiments, ± SEM. (C) Partial rescue of cell-cell contact stability by RhoGAP expression (EGFP-tail) (lower panels), but not by motor-neck expression (EGFP-Motor-Neck) (upper panels) in myosin-IXA-depleted cells. Depletion of endogenous myosin-IXA in EGFP-Motor-Neck expressing line (duplex-1) and in EGFP-tail expressing line (duplex-2). Upon cell-cell collision, cells remain attached for up to 1 h (arrow) (Movie S9). Min:sec; bar = 30 μm. (D) Quantification of rescue of cell-cell contact stability. 2 independent experiments, ± SEM. (E) Partial rescue of cell scattering phenotype in myosin-IXA-depleted cells after expression of EGFP-tail, but not EGFP-motor-neck, constructs. 2 independent rescue experiments, ± SEM. *, P = 0.03, unpaired t test.