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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Prog Retin Eye Res. 2011 Dec 22;31(2):121–135. doi: 10.1016/j.preteyeres.2011.12.001

Figure. 2.

Figure. 2

A2E accumulates in central and peripheral retina of adult human eye. RPE/choroid samples obtained from human eye using 4 mm trephine. A. RPE/choroid from central (centered on fovea) retina. Tissue was analyzed by ultra performance liquid chromatography/mass spectrometry (MS) analysis (electrospray ion multi-mode ionization, ESI). Upper trace, chromatogram with absorbance monitoring at 430 nm. Inset, absorbance spectra of A2E and isoA2E. Lower trace, ESI-MS at m/z 592, the molecular weight of A2E. B. RPE/choroid from each of four retinal quadrants. Shown are absorbance spectra and molecular weights (m/z) for A2E and isoA2E obtained by analysis of samples from each quadrant. C. Chloroform/methanol extract of human RPE/choroid showing water soluble and chloroform/methanol soluble phases.