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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Dev Biol. 2012 Jan 3;363(1):320–329. doi: 10.1016/j.ydbio.2011.12.038

Figure 1. Generation of Pax6ΔPE/ΔPE and Pax6ΔDP/ΔDP enhancer knockouts.

Figure 1

(A) A 450 bp and a 3.1 kb Pax6 genomic sequence were deleted by gene targeting in Pax6ΔPE and Pax6ΔDP, respectively. Frt and LoxP sites are represented by open arrows and solid triangles. PE, the Pax6 promoter-proximal enhancer; EE, the ectodermal enhancer; DE, the promoter-distal enhancer; Sh, Sph I; Sp, Spe I. (B) Southern Blots using the 5′ Sph I or the 3′ Spe I probes confirmed the Pax6Neo allele in the ES cell lysates and the Pax6ΔPE and Pax6ΔDP alleles in mouse tail extracts. (C) Genotyping confirmation of the Pax6ΔPE and Pax6ΔDP mice.