. Author manuscript; available in PMC: 2013 Jul 1.

Published in final edited form as: Brain Behav Immun. 2011 Oct 17;26(5):766–777. doi: 10.1016/j.bbi.2011.10.003

Table 3. IL-4 re-directed active primary microglia towards an M2 profile.

Primary microglia were collected from neonatal mice, plated, and pre-treated with saline or LPS (10 ng/mL) for 1 h. After 1 h cells were treated with vehicle or IL-4 (20 ng/mL) for an additional 3 h (n=5). RNA was isolated and M1, M2a, and M2c gene expression was determined. These results represent 2 independent experiments.

	mRNA	Saline Vehicle	Saline IL-4	LPS Vehicle	LPS IL-4

M1	iNOS	1.00 ± 0.05 (a)	0.76 ± 0.08 (b)	270.85 ± 43.97 (c)	187.95 ± 31.65 (c)
M1	IL-1β	1.03 ± 0.13 (a)	0.64 ± 0.07 (b)	114.57 ± 10.99 (c)	102.87 ± 10.7 (c)

M2	Arg	1.19 ± 0.38 (a)	123.89 ± 44.13 (b)	1.06 ± 0.43 (a)	14.35 ± 4.16 (c)
	IL-10	1.03 ± 0.11 (a)	2.71 ± 0.39 (b)	5.65 ± 0.65 (c)	7.18 ± 1.41 (c)
	SOCS1	1.00 ± 0.03 (a)	13.36 ± 0.73 (b)	23.15 ± 3.97 (c)	52.25 ± 4.99 (d)

Table represents the mean ± SEM. Means with different letters (a,b,c) are different (p<0.1) from each other. Underlined letters trend towards a difference (p<0.1) from the same letter (c, c).