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PAR-2 activation assay conducted in PathHunter eXpress™ β-arrestin PAR-2 cells. The PAR-2 AP (SLIGKV) at 10 μM activates PAR-2 whereas S100A8 or S100A9 at 10 μg/ml or the PAR-4 AP (GYPGQV) at 100 μM do not activate PAR-2 (A). S100A8 and S100A9 fail to inhibit PAR-2 activation by 10 μM PAR-2-AP. The data represent the mean ± SD and it is a representative of 3 experiments conducted in triplicates.
