Figure 1. Targeted DCs migrate through the dermis in steady state and inflamed skin.
1μg IgG2a control or anti-DEC-205 mAb were injected intradermally into the ears of C57BL/6 mice. The injection site was then topically treated with imiquimod cream or left untreated (n.t.). Four days after injection, dermal sheets were prepared and fixed. Targeted cells were detected by anti-rat IgG / Alexa Fluor 594™. Dermal lymph vessels were revealed by anti-LYVE-1 antibody, specific for lymphatic endothelial cells. Scale bar represents 100μm. Results are representative of two independent experiments.