Figure 6. Resident CD8α⁺ DCs in skin-draining LN are targeted via passive transport of intradermally injected aDEC/OVA.

1μg IgG2a control or anti-DEC-205 mAb were injected intradermally into the ears of BALB/c mice. The injection site was immediately treated with imiquimod cream, or left untreated. Targeting antibody was detected on permeabilised lymph nodes cells by anti-rat IgG / APC, 4h, 2 days or 4 days after injection of targeting antibodies, under steady state (n.t., untreated injection site) or inflamed conditions (imiq., imiquimod-treated site). CD11c⁺ CD8α⁺ DCs or CD11c⁺ CD8α^neg DCs were gated, and the proportions (A) and absolute numbers (B) of targeted cells among each population was determined. Each data point represents measurements obtained from pooled auricular lymph nodes of 1-3 mice. Results displayed are pooled from six independent experiments.