Figure 9. Depletion of Langerin⁺ DCs enhances targeting by anti-DEC-205 of Langerin^neg CD11b⁺ dermal DCs.

1μg IgG2a control or anti-DEC-205 mAb were injected intradermally into the ears of Langerin-DTR-EGFP mice, which were injected i.p. with PBS or 0.5μg DT two days before. Four hours later, the mice were sacrificed; ears and auricular lymph nodes were collected. (A) Targeting antibody was detected after permeabilisation by anti-rat IgG / APC, and the cells were counterstained with Langerin, CD8α, CD11c and CD11b. The number of targeted cells was calculated for CD8α⁺ Langerin⁺, CD8α⁺ Langerin^neg, CD8α^neg Langerin⁺ and CD11b⁺ Langerin^neg DCs. (B) Whole ear skin explants were cultured for 4 days on complete medium. Emigrating cells were collected in the supernatant. Targeting antibody was detected after permeabilisation by anti-rat IgG / APC, and the cells were counterstained with CD11b, Langerin and CD11c. Plots depicts cells gated for CD11c expression. Results are representative for three independent experiments.