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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Inflamm Bowel Dis. 2011 Nov 13;18(7):1303–1314. doi: 10.1002/ibd.21930

Figure 6.

Figure 6

DSS induces decreased matriptase in colonic T84 epithelial monolayers. Polarized T84 monolayers (start TEER ~900 Ohms.cm2) were treated with or without 5% DSS in serum-free DME for 2.5 hrs, the DSS was removed, and the monolayers cultured in serum containing media for an additional 24 hrs. (A) (Upper panel) Changes in TEER. TEER was completely abolished after 2.5 hrs of DSS treatment. Upon removal of DSS (arrow), TEER values increased and approached that of untreated cultures by 24 hrs. (Lower panel) Immunoblot analysis showing that matriptase protein expression is significantly reduced (over 90%) upon exposure to DSS, and recovers upon removal of the DSS. (B) Matriptase siRNA knockdown in T84 monolayers compromises tight junction function. (Upper panel) Immunoblot analysis showing 80% knockdown of matriptase protein expression by 3 days. (Lower panel) TEER development is impaired in matriptase silenced T84 monolayers compared with control cells (siCtl vs siM2).