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. Author manuscript; available in PMC: 2013 Feb 17.
Published in final edited form as: Circ Res. 2012 Jan 5;110(4):551–559. doi: 10.1161/CIRCRESAHA.111.255927

Figure 7. Abrogation of LR polarity and LR alignment of multicellular ridges with removal of substrate interfaces.

Figure 7

A, Microscopic images after shadow-mask plating show plating limited to rectangular domains. Scale bar, 300 μm and 200 μm (inset). B, Polarity of VMCs at the edges of cell sheets shadow-plated onto uniform FN (n = 3 experiments; > 175 cells each; mean ± s.d.) and VMCs near the FN/PEG interface with Y27632 (n = 3; > 135 cells each) or blebbistatin (n = 3; > 150 cells each) inhibition. C, Stacked images of NMM-IIa immunofluorescence after shadow-plating on FN (n = 40). Scale bar, 100 μm. D, Multicellular patterns by hematoxylin staining after shadow-plating on FN substrate. Scale bar, 2 mm.