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. Author manuscript; available in PMC: 2013 Apr 10.
Published in final edited form as: Virology. 2012 Feb 3;425(2):82–94. doi: 10.1016/j.virol.2012.01.006

Figure 3. Oas1a, Oas1b, Irf7 and Irf1 gene expression is induced in WNV Eg101-infected tSTAT1−/− and tSTAT2−/− MEFs.

Figure 3

Fold induction of (A) Oas1a, (B) Oas1b, (C) Irf7 and (D) Irf1 mRNA mRNA expression levels in t129, tSTAT1−/− and tSTAT2−/− MEF cell lines was measured by real-time qRT-PCR. Because the expression of Oas1a was not detected (ND) in the mock-treated tSTAT1−/− and tSTAT2−/− MEFs, the expression of Oas1a was normalized to the 8 h WNV Eg101 sample. Each experiment was repeated at least two times in triplicate. The mRNA level of each gene was normalized to the level of GAPDH mRNA in that sample and is shown as the fold change over the amount of mRNA in mock samples expressed in RQU. The error bars represent the calculated SEM (n = 3) and are based on an RQMin/Max of the 95% confidence level. (E) Culture fluid samples harvested at the indicated times after infection were titered for infectivity by plaque assay. Each data point is the average of duplicate titrations from at least two experiments. Error bars indicate SDM. (F) t129 and tSTAT2−/− MEF cell lysates were analyzed by Western blotting using antibodies specific for the indicated proteins. Actin was used as the loading control. The blots shown are representative of results obtained from two independent experiments.