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. Author manuscript; available in PMC: 2013 Apr 10.
Published in final edited form as: Virology. 2012 Feb 3;425(2):82–94. doi: 10.1016/j.virol.2012.01.006

Figure 4. Oas1a, Oas1b, Irf7 and Irf1 are induced in a type I IFN independent manner in MEFs infected with WNV Eg101.

Figure 4

Fold increase in (A) Oas1a, (B) Oas1b, (C) Irf7 and (D) Irf1 mRNA mRNA expression levels in p129 and pIFN alpha/beta R −/− MEFs was measured by real-time qRT-PCR. The mRNA level of each gene was normalized to the level of GAPDH mRNA in that sample and is shown as the fold change over the amount of mRNA in mock samples expressed in RQU. Because the expression of Oas1a was not detected (ND) in the mock-, IFN alpha- or IFN beta-treated pIFN alpha/beta R−/− cells, Oas1a expression in these cells was normalized to the 8 h WNV Eg101 sample. Each experiment was repeated at least two times in triplicate. The error bars represent the calculated SEM (n = 3) and are based on an RQMin/Max of the 95% confidence level. (E) Culture fluid harvested at the indicated times after infection was titered for infectivity by plaque assay. Each data point is the average of duplicate titrations from at least two experiments. Error bars indicate SDM.