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. Author manuscript; available in PMC: 2013 Apr 10.
Published in final edited form as: Virology. 2012 Feb 3;425(2):82–94. doi: 10.1016/j.virol.2012.01.006

Figure 7. IPS-1 is involved in the upregulation of Oas1a, Oas1b, Irf7 and Irf1 in MEFs infected with WNV Eg101.

Figure 7

Fold increase in (A) Oas1a, (B) Oas1b, (C) Irf7,(D) Irf1 and (E) IFN beta mRNA expression levels in pC57BL/6, pRIG-I−/−, pMDA5−/− and pIPS-1−/− MEFs was measured by real-time qRT-PCR. Each experiment was repeated at least twice in triplicate. The mRNA level of each gene was normalized to the level of GAPDH mRNA in that sample and is shown as the fold change over the amount of mRNA in mock samples expressed in RQUs. The error bars represent the calculated SEM (n = 3) and are based on an RQMin/Max of the 95% confidence level. (F) Culture fluid harvested at the indicated times after infection was titered for infectivity by plaque assay. Each data point is the average of duplicate titrations from at least two experiments. Error bars indicate SDM.