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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Clin Immunol. 2011 Dec 2;142(3):308–319. doi: 10.1016/j.clim.2011.11.007

Figure 2.

Figure 2

IL-33 was quantified by ELISA in the supernatants of cultured PBMCs or macrophages of normal subjects and MS patients. A. PBMCs were cultured in the presence of IL-2 for one week and then stimulated with Phorbol myristate acetate and and ionomycin (PMA) for 24 hours or media alone. Then IL-33 was quantified in the supernatants B. Adherent monocytes were expanded in the presences of GM-CSF for one week. Macrophages were then stimulated with LPS for 24 hours and secreted IL-33 protein levels were determined with ELISA.