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. Author manuscript; available in PMC: 2013 Feb 15.
Published in final edited form as: Cancer Res. 2011 Dec 15;72(4):1023–1034. doi: 10.1158/0008-5472.CAN-11-3647

Figure 1. Deletion of the c-jun in the mammary epithelial cells by transgenic mice.

Figure 1

(A) Schematic representation of the genomic c-jun, floxed c-jun allele and deleted c-jun is shown along with primer binding sites (arrows). (B) PCR, RT-PCR and western analyses to validate the c-jun gene excision, expression of Cre recombinase and reduced abundance of c-Jun protein following c-jun excision. (C) Immunohistochemical staining for Cre in bi-transgenic mice mammary gland section (i.e. positive cells indicated by an arrow). (D) Representative laser capture microdissection (LCM) image of a trypan blue stained mammary gland section from transgenic mice. I: Entire section before LCM, II: Section after LCM, III: Green stained micro-dissected mammary epithelial cells on LCM cap, IV: RT-PCR for assessment of c-jun, Cre and housekeeping gene control 18S expression on LCM RNA, V: qRT-PCR based quantification of relative abundance of the Cre-recombinase and c-jun mRNA transcripts following Cre mediated c-jun gene excision.