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. Author manuscript; available in PMC: 2013 Feb 15.
Published in final edited form as: Cancer Res. 2011 Dec 15;72(4):1023–1034. doi: 10.1158/0008-5472.CAN-11-3647

Figure 6. c-jun inhibits splicing activity via the dimerization domain.

Figure 6

(A) The DNT-24 luciferase β-galactosidase dual mini gene reporter system was used to transfect c-jun+/+ with c-jun−/− 3T3 cells. Data has shown as relative luciferase activity c-jun−/− MEC cells were transfected with dual splicing reporter and the data has shown as relative splicing activity. (C) Schematic representation of AP-1 transcription factor plasmids. Relative splicing activity is shown in c-jun−/− cells comparing the control vector with each of the expressed plasmids. Equimolar amounts of AP-1 protein plasmid was used to transfect c-jun−/− with c-jun+/+ 3T3 cells. (D) Schematic representation of c-jun expression plasmids with relative splicing activity shown for each of the co-transfected c-jun mutants in 3T3 cells. (E) Schematic representation of c-Fos mutant expression plasmids with relative splicing activity shown for equimolar amounts of the mutant expression plasmid in 3T3 cells. Data are mean ± SEM n>5 for each transfection.