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Multiple-label immunofluorescence visualization of c-Fos (green), glutamate (red), tyrosine hydroxylase (magenta), and DAPI nuclear stain (blue) in the RVLM of sGVS-stimulated rats. Most, but not all, of the sGVS-activated neurons in RVLM are intensely immunoreactive for tyrosine hydroxylase. The two cells indicated by white arrows in (A) are also intensely glutamate-immunofluorescent, whereas the two c-Fos-positive cells in (B) are not. Scale bars in both panels represent 20 μm.
