Fig. 3.

Conditioned medium induced differentiation in medium-density retinal cultures. Retinal cells plated at 4 × 10⁴ cells/cm² were treated with various dilutions of conditioned medium and assessed similar to Fig. 2. Note that at this cell density, no significant increase in the number of photoreceptors was seen in response to exposure to various dilutions of conditioned medium. However, cell exposure to 100% conditioned medium reflected an increase in the number of multipolar neurons reaching as high as 40% on day 1, with a simultaneous drop in the number of progenitors. This pattern was maintained on days 2 and 3 with a slight drop in the number of multipolars possibly reflecting cell death