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. 2011 Oct 28;302(3):L287–L299. doi: 10.1152/ajplung.00037.2011

Fig. 10.

Fig. 10.

PTEN effect on cell migration and mechanics is mediated by Akt and ERK signaling. A: lung bronchial (BEAS2B) epithelial cell migration was evaluated in a simplified Boyden chamber assay. The cells were seeded on the apical side of a polytetrafluoroethylene Transwell insert (8-μm pore size) and allowed to migrate for 4 h at 37°C. The cells that migrated onto the basolateral surface were dissociated with trypsin and counted. BpV (phen) treatment alone (1 μM) induced a significant increase in the number of migrating cells with respect to the control sample. Addition of either LY240092 (Akt inhibitor) or UO126 (ERK inhibitor) at 10 μM, concurrently with 1 μM bpV (phen), reduced the number of migrating cells back to the untreated, control levels. Mean values are shown ± SE; n = 20, *P < 0.05. B: BEAS2B cells were cultured in 60-mm culture dishes, and cell stiffness/Young's modulus was evaluated with AFM. 1 μM bpV (phen) treatment resulted in a decrease in the Young's modulus (i.e., decrease in cellular stiffness) with respect to the control samples, whereas the addition of LY 24009 concurrently with 1 μM bpV (phen) resulted in no change in cellular stiffness. Addition of UO126 concurrently with 1 μM bpV (phen) resulted in higher values for the Young's modulus. Mean values are shown ± SE; n = 500, *P < 0.05.