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. 2011 Sep 30;302(3):L316–L324. doi: 10.1152/ajplung.00130.2011

Fig. 4.

Fig. 4.

A: forskolin (10 μM)-stimulated adenylyl cyclase activity in cultured HASM cells induced by acute (15-min pretreatment) activation of dopamine D2 receptor by quinpirole (1 μM) (n = 13). Cells were pretreated with L-741626 (1 μM for 30-min pretreatment before quinpirole treatment) or pertussis toxin (PTX; 100 ng/ml for 4-h pretreatment before quinpirole treatment). Data represent means ± SE. **P < 0.01, compared with forskolin alone. #P < 0.05, compared with forskolin + quinpirole. B: forskolin (10 μM)-stimulated adenylyl cyclase activity in cultured HASM cells induced by chronic (1-h pretreatment) activation of dopamine D2 receptor by quinpirole (1 μM; n = 14). Cells were pretreated with L-741626 (1 μM for 30-min pretreatment before quinpirole treatment) or PTX (100 ng/ml for 4-h pretreatment before quinpirole treatment). Data represent means ± SE. **P < 0.01 and ***P < 0.001, compared with forskolin alone. #P < 0.05, compared with forskolin + quinpirole. In each experiment, values were determined in triplicate.