Fig. 3.

Tcl1 inhibits Dnmt3a enzymatic activity. (Upper) HEK 293 cells were cotransfected with DNMT3A-FLAG and either 2xHA-TCL1 or vector construct. HEK 293 cells transfected only with vector were used as a negative control. Cell lysates were immunoprecipitated for 2 h with anti-FLAG. Dnmt3a enzymatic activity was measured as described in Materials and Methods. (Lower) Amounts of Dnmt3A and Tcl1 in Dnmt3a activity assay were measured by Western blot analysis using anti-HA and anti-FLAG antibodies as indicated. The experiment was carried out in duplicate. Lanes 1 and 2 correspond to the left bar, lanes 3 and 4 correspond to the middle bar, and lanes 5 and 6 correspond to the right bar.