Fig. 1.
Effects of neonatal IH on DNA methyltransferase expression in adult rats. (A) Schematic presentation of experimental protocols. (B) Ambient O2 levels and blood O2 saturation during exposure to IH in a P5 conscious rat. (C) Body weights of P40 rats that were exposed to either room air (P40) or neonatal IH (P40IH) from ages P0 to P10. Data shown are mean ± SEM from n = 10 rats from three different litters for each group. (D) RT-qPCR analysis of Dnmt1 and Dnmt3b mRNA levels in carotid body (CB) and adrenal medulla (AM) from P40 and P40IH rats. Data are expressed relative to 18S rRNA and are presented as mean ± SEM from four independent experiments. (E) Immunoblot assays of Dnmt1 and Dnmt3b protein in adrenal medulla from P40 and P40IH rats. Densitometry data are presented as mean ± SEM from five experiments. *P < 0.05 and **P < 0.01 compared with control normoxic P40 rats.