Fig. 4.

CS790AM studies in SKH-1 mice. (A) Representative image of mice injected i.p. with vehicle, CS790AM (0.1 mM, 50 μL in 7∶3 DMSO∶PBS), CuCl₂ (5 mg/kg in 50 μL of PBS), and/or ATN-224 (5 mg/kg in 50 μL PBS). From left to right: vehicles only; vehicles and CS790AM; CuCl₂, vehicle, and CS790AM; CuCl₂, ATN-224, and CS790AM; vehicle, ATN-224, and CS790AM. For all mice, CuCl₂, ATN-224, or vehicle was injected 2 h prior to CS790AM or vehicle. Images were collected 5 min after CS790AM injection. Black arrow indicates injection location for CuCl₂, ATN-224, or vehicle; red arrow indicates injection location for CS790AM or vehicle. (B) Total photon flux from each mouse, 5 min after CS790AM injection. (C) Representative images of livers from SKH-1 mice injected i.p. with CuCl₂ or PBS, as described above, 2 h prior to CS790AM. (D) Total photon flux from imaged livers. (E) Fluorescence curves over 72 h for mice injected i.p. with CuCl₂ (black line) or PBS (gray line), as described above, 2 h prior to CS790AM. Statistical analyses were performed with a two-tailed Student’s t-test. *P < 0.05 [n = 3 (B and D), n = 4 (E)] and error bars are ± SD.