Fig. 2.

In vivo potential of transplanted progenitor cells. One thousand GFP-marked cells (12) from purified cell populations were transplanted into sublethally irradiated recipients (SI Materials and Methods). (A) Percentage contribution to erythrocytes and platelets in peripheral blood from GFP-marked donor-derived cells on days 4, 7, and 12 after transplantation. Data shown are means ± SD from two or three independent donors each into three or four recipients. y axis is log₂ scale. (B) Analysis of Lin⁻cKit⁺ bone marrow 4 and 7 d after transplantation with GFP-marked donor PreGM or BEMP cells. Formation of GMP, CMP, and MEP (Upper) and PreCFU-E, CFU-E (Lower) progenitor populations. Red, donor cells; gray, recipient cells. Representative analysis from three independent donors is shown. (C) Expression of PU.1^gfp (7) in arbitrary mean fluorescence intensity (MFI) units for each progenitor cell population indicated (Table 2 and Table S1). (Left) MFI means ± SD from four mice for each population. (Right) Representative MFI profiles. P < 0.0001 by repeated-measures ANOVA of all progenitor populations. Tukey's multiple comparison of means; P < 0.05 for comparison between all populations except between BEMPs and CD150⁺CD9^hiendoglin^lo cells. NS, not significant.