Table 1.
CFC of fractions of Lin−cKit+IL7Rα− FcγRII/IIIloendoglinlo adult bone marrow
Colonies per 100 cells plated |
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Fraction | Blast | G | GM | M | Eo | Meg | % cKit+ fraction | Meg contribution (%) |
Sca1−CD48+CD150− | 2 ± 1 | 14 ± 5 | 9 ± 2 | 10 ± 1 | 0 ± 0 | 7 ± 1 | 36 ± 2 | 21 |
Sca1−CD48+CD150+ | 0.2 ± 0.2 | 0.1 ± 0.1 | 0.0 ± 0.1 | 0.3 ± 0.2 | 0 ± 0 | 24 ± 1 | 37 ± 2 | 68 |
Sca1+ | 18 ± 5 | 1 ± 1 | 3.5 ± 4 | 9 ± 4 | 0 ± 0 | 3 ± 2 | 19 ± 1 | 11 |
Mean colony counts ± SD per 100 cells from four replicate experiments using a combined stimulus of SCF (100 ng/mL), IL-3 (10 ng/mL), and EPO (4 IU/mL). Eo, eosinophil; G, granulocyte; GM, granulocyte-macrophage; M, macrophage; Meg, megakaryocyte colonies, including colonies of megakaryocytes and erythroid cells; Meg contribution, percentage contribution to megakaryocyte CFC of each fraction, calculated by adjusting contribution to percetage of cKit+ fraction by relative ability of cells to form megakaryocyte colonies; % cKit+ fraction, percentage contribution of each fraction to Lin−cKit+ IL7Rα−FcγRII/IIIloendoglinlo bone marrow with means ± SD from four mice (Fig. S1).