Table 2.
Clonogenic colonies per 100 cells plated |
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Fraction | Blast | G | GM | M | Eo | Meg | % of CD150+ fraction | Meg contribution (%) |
CD9loendoglinlo (BEMP) | 0.5 ± 0.5 | 0.2 ± 2 | 0.2 ± 0.2 | 0.2 ± 0.2 | 0 ± 0 | 28 ± 9 | 51 ± 4.9 | 92 |
CD9hiendoglinlo | 0.5 ± 0.6 | 0.1 ± 0.2 | 0.2 ± 0.4 | 0.3 ± 0.3 | 0 ± 0 | 10 ± 4 | 13 ± 2.3 | 8 |
CD9loendoglinhi (PreCFU-E) | 0 ± 0 | 0 ± 0 | 0.2 ± 0.3 | 0 ± 0 | 0 ± 0 | 0.5 ± 0.3 | 36 ± 5.8 | 0 |
Mean colony counts ± SD per 100 cells from four replicate experiments using a combined stimulus of SCF, IL-3, and EPO. Eo, eosinophil; G, granulocyte; GM, granulocyte-macrophage; M, macrophage; Meg, megakaryocyte colonies, including colonies of megakaryocytes and erythroid cells; Meg contribution, percentage contribution to megakaryocyte CFC of each fraction, calculated by adjusting contribution to percentage of CD150+ fraction by relative ability of cells to form megakaryocyte colonies; % of CD150+ fraction, percentage contribution each population to Lin−cKit+Sca1−IL7Rα−FcγRII/IIIloCD150+ adult bone marrow with means ± SD from six mice.