Table 1.
Transgene | Genotype | No. of plants | Pollen fertility, % | Spikelet fertility, % |
Actin:NT1 (T0) | Positive | 19 | 48.7 ± 6.72 | |
Negative | 7 | 2.9 ± 1.00 | ||
t | 6.75 | |||
P | 0.0000 | |||
Actin:ST1 (T0) | Positive | 14 | 46.6 ± 5.51 | |
Negative | 5 | 3.2 ± 1.82 | ||
t | 7.48 | |||
P | 0.0000 | |||
Actin:NT1 (T1) from three families | Positive | 58 | 83.7 ± 1.03 | 88.0 ± 1.00 |
Negative | 26 | 5.1 ± 0.74 | 5.6 ± 0.83 | |
t | 61.67 | 63.13 | ||
P | 0.0000 | 0.0000 | ||
Actin:ST1 (T1) from three families | Positive | 58 | 80.8 ± 1.08 | 79.3 ± 1.85 |
Negative | 25 | 4.3 ± 0.99 | 4.7 ± 0.94 | |
t | 52.16 | 35.96 | ||
P | 0.0000 | 0.0000 | ||
Actin:NT1-mORF (T0) | Positive | 14 | 43.6 ± 8.18 | |
Negative | 8 | 2.9 ± 1.49 | ||
t | 3.70 | |||
P | 0.0007 | |||
Actin:NT1-ORF (T0) | Positive | 17 | 7.4 ± 1.97 | |
Negative | 1 | 1.3 | ||
t | 0.72 | |||
P | 0.2300 | |||
Actin:NT1-△SNP (T0) | Positive | 15 | 7.6 ± 2.84 | |
Negative | 2 | 0.4 ± 0.36 | ||
t | 0.91 | |||
P | 0.1891 | |||
Actin:NT1-△110bp (T0) | Positive | 17 | 40.7 ± 7.47 | |
Negative | 4 | 4.8 ± 4.65 | ||
t | 2.27 | |||
P | 0.0177 |
All data are given as mean ± SEM. P values were obtained by t tests between positive and negative plants.