Fig. 2.

Four types of auditory responses from AMMC PNs. (A) Gal4 expression patterns reported by UAS-mCD8::GFP (green) in the brain immunostained with anti-Discs large (DLG) antibody (magenta). VT30609-Gal4 contains a ventral tract (arrow) that is not in the other drivers. Arrowheads denote cell bodies. (B) Calcium responses in the IVLP in Gal4s>UAS-GCaMP1.6 flies. Each value represents mean ± SEM (n = 5–8) for the ratio of peak amplitudes before and after acoustic stimuli (ΔF/F%; *P < 0.05, **P < 0.01, and ***P < 0.001). (C) Axon-dendrite polarity analysis in Gal4s>UAS-Dscam::GFP;UAS-mKO and Gal4s>UAS-syt::GFP;UAS-mKO flies. In all cases, AMMC PNs project Dscam fibers into the AMMC (yellow dotted circle) and synaptotagmin fibers into the IVLP (white dotted circle). Green, GFP; magenta, mKO. (Scale bars, 50 μm.)