Fig. 3.

In vivo phenotypic analysis of the chimeric S. cerevisiae PCNA variants from group I and group II species. (A) Sensitivity of yeast strains containing chimeric PCNA variants as the sole source of PCNA to DNA-damaging agents, including HU (Center) and MMS (Right). (B) Nonviable strains containing chimeric ScPCNA with the N. crassa or A. nidulans IDCL sequence. Yeast strains containing ScPCNA expressed from a URA3 plasmid and chimeric Scpcna genes expressed from a LEU2 plasmid are viable (Center). On selection for loss of WT PCNA using 5-FOA, chimeric PCNA strains are deemed nonviable (Right).