Skip to main content
. 2012 Jan 17;109(7):E406–E414. doi: 10.1073/pnas.1108633109

Fig. 4.

Fig. 4.

In vivo phenotypic analysis of WT PCNAs from group I and group II species. (A) HU (Center) and MMS (Right) sensitivity of yeast strains containing the ScPCNA, KwPCNA, AgPCNA, or DhPCNA variants as the sole source of PCNA. (B) Nonviable strains containing NcPCNA. (C) Nonviability of the S. cerevisiae strain containing YlPCNA is suppressed by the replacement of the native IDCL region with the ScPCNA (POL30) IDCL region (Ylpcna-POL30-IDCL), indicating the importance of this region in facilitating strain survival (the generation of S. cerevisiae strains containing PCNA variants as a sole source of PCNA in the cell was performed as described in Fig. 3 and in the main text). (D) HU (Center) and MMS (Right) sensitivity of strains containing the SpPCNA the chimeric Ylpcna-POL30-IDCL, or the chimeric pcn1-POL30-IDCL gene. The sensitivity of the SpPCNA-containing strain is suppressed by replacement of its native IDCL region with the POL30 IDCL region (pcn1-POL30-IDCL).