Fig. 1.

Lkb1 is required for mammary epithelial integrity. (A) Western blot showing loss of Lkb1 protein in isolated Lkb1^lox/lox MMECs 72 h after AdCre recombinase infection. (B) Morphology of the AdCre-infected Lkb1^lox/lox MMECs grown in 3D culture for 5 d. (Scale bar: 100 μm.) (C) Day 10 Lkb1^lox/lox 3D acini immunostained for Par-3 and GM-130. (Scale bar: 20 μm.) (D) Day 10 Lkb1^lox/lox 3D acini immunostained for E-cadherin and ZO-1. Asterisks denote the luminal side in close-up images. (Scale bar: 20 μm.) (E) TEM images of control and AdCre-infected Lkb1^lox/lox 3D acini. The red arrowheads mark the ends of TJs, and the black arrowhead denotes a desmosome. (Scale bar: 0.5 μm.) (F) Quantification of TJ length. Mean and SD values were calculated from at least three independent experiments.