Fig. 2.

Loss of Lkb1 results in spontaneous branching and disruption of BM. (A) Phase-contrast images of day 10 control and Lkb1^lox/lox 3D acini showing branching. Percentages indicate the number of acini with branching structures. (Scale bar: 100 μm.) (B) Z-stack of confocal images to visualize hollow cavities in branching Lkb1^lox/lox 3D acini. The mitotic activity is visualized with phospho-Histone H3 antibodies. (Scale bar: 20 μm.) (C) SEM images of AdCre-infected control and Lkb1^lox/lox acini grown on top of Matrigel. (Scale bars: 50 μm in images with a magnification of 500×, 20 μm in images with a magnification of 2,000×.) (D) Day 10 control and AdCre-infected Lkb1^lox/lox 3D acini immunostained for Laminin-332, nidogen, or Collagen IV show a discontinuous staining pattern surrounding LKB1-lacking acini. (Scale bar: 20 μm.)