Fig. 4.

c-Myc and loss of Lkb1 cooperate in mammary tumorigenesis. (A) Kaplan–Meier survival analysis of Myc and Myc/Lkb1- mice. The arrow approximates the first pregnancy. (B) Photographs of mammary tumors in W-Myc;W-Cre and Myc/Lkb1- mice. Note a solitary single tumor in the W-Myc;W-Cre mouse and the multiplicity of tumors in the two Myc/Lkb1- mice. (C) Tumor multiplicity (Left) and volume (Right) in the mice. (D) Tumor histopathological findings. Shown is an H&E-stained adenocarcinoma from a Myc mouse as well as adenocarcinoma and MIN from Myc/Lkb1- mice. The large magnification shows nuclear pleomorphism and cellular atypia identical in all tumors. However, the adenocarcinoma-defining invasive front was absent in MIN (images with magnifications of 100× and 40×). (Scale bars: 200 μm in images with a magnification of 100×, 50 μm in images with a magnification of 400×.)